peptide linkage การใช้

• Carboxypeptidase cleaves peptide linkages during digestion of proteins.
• It is active on peptide linkages involving the carboxyl group of lysine or arginine.
• This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.
• In addition, a second hydrogen bond is formed between the tyrosine and a peptide linkage of longer peptide substrates.
• DAP is often found in the peptide linkages of NAM-NAG chains that make up the cell wall of gram-negative bacteria.
• Glutathione is capable of preventing damage to important amine group of cysteine, and the carboxyl group of cysteine is attached by normal peptide linkage to a glycine.
• The modified intein undergoes a self-cleavage reaction at its N-terminal peptide linkage with 1, 4-dithiothreitol ( DTT ), ?-mercaptoethanol ( ?-ME ), or cystine at low temperatures over a broad pH range.